k222 rHev b 9 Latex
Allergens within Occupational Allergens
- Latin name: Hevea brasiliensis
- Common names: Enolase
- Source material: Recombinant non-glycosylated MBP fusion protein produced in an E. coli strain carrying a cloned cDNA encoding Hevea brasiliensis allergen Hev b 9.
Recombinant allergen
Hevea brasiliensis allergen Hev b 9.
Biological function
Enolase
Mw
51 kDa
Allergen description
Hev b 9 (1) is an enolase, an ubiquitous enzyme involved in the carbohydrate catabolism pathway. A sequence identity of about 60% was reported between the enolase of H. brasiliensis and the enolases of moulds, and, in particular, cross-reactivity was demonstrated with the enolases from Cladosporium herbarum and Alternaria alternata (1). With the use of recombinant Hev b 9 (rHev b 9), IgE-binding reactivity was observed in 16 out of 110 Latex-allergic adults (14.5%). In a recent study, only 1 out of 40 health care workers tested, and no patient with spina bifida, was reported to have Hev b 9- specifc IgE antibodies (2).
References:
- Wagner S, Breiteneder H, Simon-Nobbe B, Susani M, Krebitz M, Niggemann B, et al. Hev b 9, an enolase and a new cross-reactive allergen from hevea latex and molds. Purification, characterization, cloning and expression. Eur J Biochem 2000;267(24):7006-7014.
- Rihs H-P, Raulf-Heimsoth M. Natural rubber latex allergens: Characterization and evaluation of their allergenic capacity. New Horizons, Pharmacia Diagnostics AB 2003;No 3.
2006