How does the temperature effect ECP levels?

ImmunoCAP ECP

Print Print icon Tell a friend Tell a friend icon
 
The temperature during the clotting process affects the ECP values.
Higher temperatures stimulate the eosinophils to an increased release of ECP. ECP values increase by about 10 - 15% per 1°C.
 
To obtain reproducible and comparable results, the clotting temperature should be as stable as possible during the clotting process and between different sampling occasions.
 
Recommended Clotting Temperature: + 20 - 24°C.

References
  1. Ahlstedt S, Enanader I, Peterson CG, Lanner Å. The clinical assessment of the inflammatory component in asthma with emphasis on the eosinophils. Pract Allergy Immunol 1993;8:149-154.
  2. Reimert CM, Poulsen LK, Bindslev-Jensen C, Kharazmi A, Bendtzen. Measurement of eosinophil cationic protein (ECP) and eosinophil protein X/eosinophil derived neurotoxin (EPX/EDN). J Immunol Methods 1993;166:183-190.
  3. Pronk-Admiraal CJ, Bartles PC. Effect of clotting temperature and eosinophil concentration on the eosinophil cationic protein concentration in serum. Scand J Clin Lab Invest 1994;54:185-188.
 
Further Reading