Expected Values

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Examples of results obtained with ImmunoCAP Specific IgG4 when assaying 100 healthy non-allergic blood donors with 10 antigens are shown in fig. 1-2.
 

 

AGf76

AGf77

AGf78

Gf1

Max

15.6

38.1

11.8

7.4

95-Percentile

3.6

9.6

5.6

4.0

Median

0.13

1.3

0.86

0.35

 

 

Gd1

Gd2

Gg6

Gi1

Gi3

Gt3

Max

0.37

0.30

0.34

0.18

0.29

0.13

95-Percentile

0.31

0.22

0.17

0.12

0.08

0.07

Median

0.16

0.10

0.06

0.05

0.03

0.01

 
Precision
 
 

Coefficients of variation (%)

Sample level
mgA/l

Within assay

Between assay

0.16-0.59

8.1

8.8

1.6-12.2

6.3

10.0

19.7

10.0

6.9

 
Representative antigens from 5 antigen groups have been tested. Each sample has been assayed in 3 replicates on totally 18 different occasions using the same lot of antigen, performed with3 ImmunoCAP 100 instruments using stored calibration curves.The samples have been diluted by the ImmunoCAP 100 instrument.The coefficient of variation (%) for sample dilution is approx. 4% and is included in the coefficients of variation shown above.