Sample Collection

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Blood collection
Blood should be collected by venipuncture, allowed to clot, and the serum separated by centrifugation. Samples should preferably be taken between 15 minutes and 3 hours after the event suspected of causing mast cell activation.
 
Increased levels of tryptase can usually be detected up to three to six hours after the reaction. They return to normal within 12-14 hours after release (1).
 
Serum sample storage
Specimens may be kept at room temperature (RT) for shipping purposes for 2 days.
Store at 2-8°C if assayed within 5 days after collection.
For longer periods, store samples at -20°C or -70°C.
 
Plasma samples
Plasma samples have been assayed and found to give comparable results to serum samples.
 
Nasal lavage
Diluted and undiluted nasal lavage samples can be used in ImmunoCAP Tryptase. For dilution use ImmunoCAP IgE/ECP/Tryptase Diluent. There are several methods for collection of nasal lavage described in the literature. Analyze the collected sample in ImmunoCAP Tryptase according to the Directions for Use. 

References:

    1. Schwartz LB, Yunginger JW, Miller J, Bokhari R and Dull D: Time course of appearance and disappearance of human mast cell tryptase in the circulation after anaphylaxis. J Clin Invest 1989;83:1551-1555.