Product Description

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Test Principle
 
Anti-IgE, covalently coupled to ImmunoCAP, reacts with the total IgE in the patient serum sample.
After washing, enzyme-labelled antibodies against IgE are added to form a complex.
After incubation, unbound enzyme-labelled anti-IgE is washed away and the bound complex is then incubated with a developing agent.
After stopping the reaction, the fluorescence of the eluate is measured. The fluorescence is directly proportional to the concentration of IgE in the serum sample.
 
Patient Sample
Patient samples are run in single determinations and evaluated against the stored calibration curve.
 
Measuring Range
Undiluted serum 2 - 5000 kUA/l.
The range can be extended by sample dilution with ImmunoCAP IgE / ECP / Trypthase Sample Diluent.
 
Precaution
As in all diagnostic testing a definitive clinical diagnosis should not be based on any single diagnostic method. It should be made by the physician after evaluation of all clinical and laboratory findings.